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Details van artikel 8 van 21 gevonden artikelen
| Titel: |
Creating hybrid genes by homologous recombination |
| Auteur: |
Peter L. Wang |
| Verschenen in: |
Disease markers |
| Paginering: | Jaargang 16 (2001) nr. 1-2 pagina's 3-13 |
| Jaar: |
2001-04-01 |
| Inhoud: |
Recombination of homologous genes is a powerful mechanism for generating sequence diversity, and can be applied to protein analysis and directed evolution. {\it In vitro} recombination methods such as DNA shuffling are very flexible and can give hybrid genes with multiple crossovers; they have been used extensively to evolve proteins with improved and novel properties. {\it In vivo} recombination in both {\it E. coli} and yeast is greatly enhanced by double-strand breaks; for {\it E. coli}, mutant strains are often necessary to obtain high efficiency. Intra- and inter-molecular recombination {\it in vivo} have distinct features; both give hybrids with one or two crossovers, and have been used to study structure-function relationships of many proteins. Recently {\it in vivo} recombination has been used to generate diversity for directed evolution, creating a large phage display antibody library. Recombination methods will become increasingly useful in light of the explosion in genomic sequence data and potential for engineered proteins. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 8 van 21 gevonden artikelen
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