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Details for article 7 of 21 found articles
| Title: |
Comparison between hybridoma and Fab/phage anti-RhD: Their V gene usage and pairings |
| Author: |
W.S. Perera M.T. Moss S.J. Urbaniak |
| Appeared in: |
Disease markers |
| Paging: | Volume 16 (2001) nr. 1-2 pages 15-19 |
| Year: |
2001-04-01 |
| Contents: |
Our 11 anti-RhD's in conjunction with 37 previously published RhD antibodies, produced by hybridoma technology were analysed for germline gene usage and restriction in V_{\rm H} and V_{\rm L} pairings. The 17 V_{\rm H}germline genes used by the hybridoma anti-RhD IgG were derived from 4 VH families (VH1, VH2, VH3 and VH4). Eighteen kappa chains were restricted to only 5 germline genes from only 2 V\kappafamilies (V\kappa1 and \kappa3). However, the 13 lambda chains were not as restricted, using 10 V_{\lambda} germline genes from 4 families (V\lambda1, V\lambda2, V\lambda3 and V\lambda8). Fifty six unique Fab/phage anti-RhD were also analysed. In all cases the Fab/phage V_{\rm H} germline genes were derived from the VH3 family (41/41). The 29 kappa chains were restricted to 4 germline genes primarily from V\kappa1 (97%) germline genes from 5 families (V\lambda1, V\lambda2, V\lambda3, V\lambda4 and V\lambda7). The V_{\rm H} germline genes of the Fab/phage were restricted to 4 of the 17 used by the hybridoma anti-RhD IgG (DP46, DP49, DP50 and DP77). Ninety percent of the Fab/phage were restricted to 1 of the 5 V_{\kappa}germline genes used by the IgG (DPK9). However, the repertoire of the V_{\lambda}germline genes used in these two systems is different, with analysis showing greater diversity in V_{\lambda} gene usage with 8 unique germline genes used by 76% Fab/phage compared to 4 unique genes used by 46% hybriboma anti-RhD. |
| Publisher: |
IOS Press |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 7 of 21 found articles
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