Bioencapsulation of Florfenicol in Brine Shrimp, Artemia Franciscana, Nauplii
Titel:
Bioencapsulation of Florfenicol in Brine Shrimp, Artemia Franciscana, Nauplii
Auteur:
Irja Sunde Roiha Erling Otterlei Ole Bent Samuelsen
Verschenen in:
Journal of bioanalysis & biomedicine
Paginering:
Jaargang 02 (2010) nr. 03 pagina's 060-064
Jaar:
2010
Inhoud:
The brine shrimp <italic>Artemia franciscana</italic> is one of the most common live-feed organisms for use in the larval culture of marine fish production. Bioencapsulation of florfenicol, an antibacterial agent, in <italic>Artemia nauplii</italic> was investigated as a potential carrier for this drug to marine larvae. Florfenicol was delivered directly to the organisms as particles, andthe doses ranged from 100 to 2000 mg/l. Analysis of florfenicol concentrations in <italic>Artemia</italic> sp. nauplii were performed using high performance liquid chromatography (HPLC). The uptake of florfenicol in <italic>Artemia nauplii</italic> increased with particle size, dose, and exposure time, obtaining the highest concentration of 5.02 ng/nauplius, using a dose of 300 mg/l AQUAFLOR premix and 10 min exposure time. However, to obtain reproducible results, an enrichment time of at least 60 min is recommended.