Bioencapsulation of Florfenicol in Brine Shrimp, Artemia Franciscana, Nauplii
Title:
Bioencapsulation of Florfenicol in Brine Shrimp, Artemia Franciscana, Nauplii
Author:
Irja Sunde Roiha Erling Otterlei Ole Bent Samuelsen
Appeared in:
Journal of bioanalysis & biomedicine
Paging:
Volume 02 (2010) nr. 03 pages 060-064
Year:
2010
Contents:
The brine shrimp <italic>Artemia franciscana</italic> is one of the most common live-feed organisms for use in the larval culture of marine fish production. Bioencapsulation of florfenicol, an antibacterial agent, in <italic>Artemia nauplii</italic> was investigated as a potential carrier for this drug to marine larvae. Florfenicol was delivered directly to the organisms as particles, andthe doses ranged from 100 to 2000 mg/l. Analysis of florfenicol concentrations in <italic>Artemia</italic> sp. nauplii were performed using high performance liquid chromatography (HPLC). The uptake of florfenicol in <italic>Artemia nauplii</italic> increased with particle size, dose, and exposure time, obtaining the highest concentration of 5.02 ng/nauplius, using a dose of 300 mg/l AQUAFLOR premix and 10 min exposure time. However, to obtain reproducible results, an enrichment time of at least 60 min is recommended.