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| Titel: |
Bioremediation of Trichlorpyr Butoxyethyl Ester (TBEE) in bioreactor using adapted Pseudomonas aeruginosa in scale up process technique |
| Auteur: |
MH Fulekar M Geetha J Sharma |
| Verschenen in: |
Biology and medicine |
| Paginering: | Jaargang 1 (2009) nr. 3 pagina's 1-6 |
| Jaar: |
2009 |
| Inhoud: |
Pseudomonas aeruginosa has been adapted at varying concentrations of pesticide - Trichlorpyr ButoxyEthyl Ester (TBEE) viz. 10,25 50, 75 and 100 mg/l in MSM using incubator shaker at 37 ºC and 150 rpm byscale up process technique. In the beginning 10 mg/l concentration of TBEE was supplied to microorganismin minimal salt medium (MSM) under controlled conditions for 14 days. The culture was subsequently scaledup to higher concentration of TBEE by transferring one milliliter MSM containing 10 mg/l to 25 mg/lconcentration in MSM and incubated for continuous 14 days. The Pseudomonas aeruginosa (NCIM 2074)was adapted in increasing concentration of TBEE at 50 mg/l, 75 mg/l and 100 mg/l; after every 14 daysusing a total period of 70 days. During adaptation period, the pseudomonas aeruginosa degraded the TBEEcompletely at concentrations of 10 mg/l and 25 mg/l; while 49% and 23% degradation in 75 mg/l and 100mg/l of TBEE was observed. Hence bioremediation studies has been carried out at concentrations 10, 25 50mg/l of TBEE using flask shake method. GCMS data showed that TBEE degraded 100% within 3 days at 10mg/l concentration and 5 days at 25 mg/l initial concentrations. While in case of 50 mg/l initial concentrationof TBEE the compound was found persisting till 8 days period. The major intermediated, during thebioremediation of TBEE, were found to be 3,5,6-trichloro-2-pyridinol and 2,4,6-trichloro benzene amine.These intermediates are less toxic than parent compounds which on long term acclimatization would convertinto environment friendly compounds. |
| Uitgever: |
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| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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