Digitale Bibliotheek
Sluiten Bladeren door artikelen uit een tijdschrift
 
<< vorige    volgende >>
     Tijdschrift beschrijving
       Alle jaargangen van het bijbehorende tijdschrift
         Alle afleveringen van het bijbehorende jaargang
           Alle artikelen van de bijbehorende aflevering
                                       Details van artikel 3 van 14 gevonden artikelen
 
 
  Effect of Methomyl on the Phenobarbital and Benzo [a] Pyrene Induced Hepatic Microsomal Mixed Function Oxidase System in Rats
 
 
Titel: Effect of Methomyl on the Phenobarbital and Benzo [a] Pyrene Induced Hepatic Microsomal Mixed Function Oxidase System in Rats
Auteur: Jyotsna A. Patil1, Arun J. Patil1*, Ajit V. Sontakke1, Satish D. Kalme2 and Sanjay P. Govindwar3
Verschenen in: Al Ameen journal of medical sciences
Paginering: Jaargang 04 (2011) nr. 02 pagina's 144-151
Jaar: 2011
Inhoud: Methomyl (Lannate) is a pesticide widely used to control of insects in grape gardens. Methomyl treatment induces significant alteration in mixed function oxidase system. The present work was designed to study the inhibitory effect of methomyl on different forms of cytochrome P450 induced by phenobarbital (CYP 2B1, 2B2 and 3A) and benzo[a]pyrene induced (CYP 1A1). Adult male rats were divided into 8 groups of 6 animals each. Microsomes were isolated by calcium precipitation. The levels of electron transport components, CYP 450, cytochrome b5, and cytochrome c-reductase were determined using extinction coefficients. Activities of drug-metabolizing enzymes were assayed. Inducers like phenobarbital, benzo[a]pyrene, showed significant induction of mixed function oxidase in rat. The methomyl treatment (4mg/kg) of inducer-(Phenobarbital, benzop [a] pyrene) pretreated rat caused a significant decrease in electron transport components and activities of drug-metabolizing enzymes when compared with treatment of inducer alone. Induction of mixed function oxidase enzymes due to phenobarbital was also altered by the pretreatment of methomyl. Benzo[a]pyrene treatment of methomyl pretreated rats showed significant decreased levels of electron transport components and drug metabolizing enzymes as compared to benzo[a]pyrene treatment alone. These results indicate that the susceptibility of phenobarbital and benzo[a]pyrene induced cytochrome P450 isoform (CYP 2B1, 2B2, 3A; and CYP 1A1) to methomyl and also affected in the induction pattern of some of the inducers with respect to CYP 450 isoforms.
Uitgever: Dr.B.S.Patil (provided by DOAJ)
Bronbestand: Elektronische Wetenschappelijke Tijdschriften
 
 

                             Details van artikel 3 van 14 gevonden artikelen
 
<< vorige    volgende >>
 
 Koninklijke Bibliotheek - Nationale Bibliotheek van Nederland