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| Titel: |
Progress in Xenotransplantation Research Employing Transgenic Pigs |
| Auteur: |
H. Niemann W. A. Kues |
| Verschenen in: |
Transplantationsmedizin |
| Paginering: | Jaargang 15 (2003) nr. 1 pagina's 3-14 |
| Jaar: |
2003 |
| Inhoud: |
Microinjection of foreign DNA into pronuclei of a fertilized oocyte has predominantly been used for the generation of transgenic livestock. This technology works reliably, but is inefficient and results in random integration and variable expression patterns in the transgenic offspring. Nevertheless, remarkable achievements have been made with this technology with regard to xenotransplantation. Transgenic pigs that express human complement regulating proteins have been tested in their ability to serve as donors in human organ transplantation (i.e. xenotransplantation). In vitro and in vivo data convincingly show that the hyperacute rejection response can be overcome in a clinically acceptable manner by successfully employing this strategy. The recent developments in nuclear transfer and its merger with the growing genomic data allow targeted and regulatable transgenesis. Systems for efficient homologous recombination in somatic cells are being developed and the first knock-out pigs, carrying a deletion in the a-galactosyltransferase gene, were recently generated. It is anticipated that poly-transgenic pigs will be available as donors for functional xenografts within a few years. Similarly, pigs may serve as donors for a variety of xenogenic cells and tissues. The availability of these technologies is essential to maintain "genetic security" and to ensure absence of unwanted side effects. |
| Uitgever: |
Pabst Science Publishers |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 8 van 9 gevonden artikelen
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