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Details for article 11 of 13 found articles
| Title: |
pRS4: UN VECTOR DE CLONACION IDONEO PARA BACTERIAS ACIDO-LACTICAS DE USO ALIMENTARIO pRS4: AN APPROPRIATE CLONING VECTOR FOR LACTIC-ACID BACTERIA OF FOOD USE |
| Author: |
Mesas, J. M. Rodriguez, M. C. Alegre, M. T. |
| Appeared in: |
CyTA: journal of food |
| Paging: | Volume 5 (2006) nr. 2 pages 118-123 |
| Year: |
2006-07-01 |
| Contents: |
The ability of pRS4, a cryptic plasmid from Pediococcus pentosaceus, as a selective cloning vector for lactic acid bacteria has been analysed. The results indicate that pRS4C1, a cloning vector from pRS4 can stably transform Lactobacillus plantarum, Pediococcus acidilactici and P. pentosaceus (three lactic acid bacteria used as starters in the food industry) but it does not transform Enterococcus faecalis (a lactic acid bacteria which is undesirable due to its potential origin, mainly of faecal source). Further modifications of the pRS4C1, consisting in positional changes of the chloramphenicol resistance marker and/or single mutation into the initiation codon of the gene of the replication protein, does not generate expression of pRS4 into E. faecalis, however the ability of these derivates to transform Lactobacillus and Pediococcus remains unaltered. These results indicate that pRS4 can be used as a selective cloning vector for genetic manipulation of useful lactic acid bacteria, without risk of horizontal transfer of genetic material to undesirable lactic acid bacteria that could coexist with the formers. Se ha analizado la posibilidad de utilizar pRS4, un plasmido criptico de Pediococcus pentosaceus, como vector selectivo de clonacion en bacterias acido-lacticas. Los resultados obtenidos indican que pRS4C1, vector de clonacion derivado de pRS4, transforma en forma estable Lactobacillus plantarum, Pediococcus acidilactici y P. pentosaceus, bacterias acido-lacticas utilizadas como cultivos iniciadores en la industria alimentaria, pero no transforma Enterococcus faecalis, una bacteria acido-lactica cuya presencia en alimentos es considerada indeseable por su habitual procedencia de origen fecal. Posteriores modificaciones de pRS4C1, consistentes en cambios de posicion del marcador de resistencia a cloranfenicol y/o mutacion puntual del codon de iniciacion del gen que codifica para la proteina de replicacion, no subsanan la carencia de expresion de pRS4 en E. faecalis, pero mantienen inalterable su capacidad de transformar y expresarse establemente en las especies de Lactobacillus y Pediococcus arriba citadas. Estos resultados indican que pRS4 puede ser utilizado como vehiculo de clonacion selectivo para la manipulacion genetica de bacterias acido-lacticas utiles, sin riesgo de transferencia horizontal de material genetico a determinadas bacterias acido-lacticas indeseables, que puedan coexistir con las primeras en un mismo alimento. Palabras clave: Bacterias acido-lacticas, plasmidos, vectores de clonacion |
| Publisher: |
Taylor & Francis |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 11 of 13 found articles
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