|
| << vorige | volgende >> |
Tijdschrift beschrijving
Alle jaargangen van het bijbehorende tijdschrift
Alle afleveringen van het bijbehorende jaargang
Alle artikelen van de bijbehorende aflevering
Details van artikel 4 van 10 gevonden artikelen
| Titel: |
Comparison of different extraction and clean-up procedures for the determination of fumonisins in maize and maizebased food products |
| Auteur: |
De Girolamo, Annalisa Solfrizzo, Michele Visconti, Angelo Von Holst, Christoph |
| Verschenen in: |
Food additives and contaminants. Pt. A, Chemistry, analysis, control, exposure & risk assessment |
| Paginering: | Jaargang 18 (2001) nr. 1 pagina's 59-67 |
| Jaar: |
2001-01-01 |
| Inhoud: |
In order to optimize the analytical method for the determination of fumonisin B1 (FB1) and fumonisin B2 (FB2) in diVerent maize products, five materials (maize flour, cornflakes, extruded maize, muffins and infant formula) were investigated under a variety of experimental conditions organized in a ruggedness test according to a factorial design. The influence of five factors (extraction solvent, extraction mode, volume of extraction solvent, test sample size and clean-up) on method performances was tested by four laboratories using spiked materials (0.5 μg/g and 1.5 μg/g FB1 + FB2) and naturally contaminated materials (ca 1.5 μg/g with FB1 + FB2). The end determination step was performed by high-performance liquid chromatography analysis of the o-phthaldialdehyde derivatized extracts. The ruggedness test permitted identification of two critical factors in the analysis of fumonisins in the above products, namely 'extraction solvent' and 'cleanup procedure'. In particular, the use of acetonitrile (ACN)-water (1 + 1, v + v) as extraction solvent and immunoaffinity column for clean-up provided better recovery of fumonisins and chromatographic resolution as compared with methanol (MeOH)-water (3 + 1, v + v) and strong anion exchange (SAX), respectively. However, phase separation occurring after extraction with ACN-water may have given incorrect results. Based on the information obtained with the present study it was possible to develop a new method horizonhorizontally applicable to all the above mentioned maize-based food matrices. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 10 gevonden artikelen
| << vorige | volgende >> |