A survey of zearalenone in corn using Romer Mycosep™ 224 column and high performance liquid chromatography
Titel:
A survey of zearalenone in corn using Romer Mycosep™ 224 column and high performance liquid chromatography
Auteur:
Silva, C. M. G. Vargas, E. A.
Verschenen in:
Food additives and contaminants. Pt. A, Chemistry, analysis, control, exposure & risk assessment
Paginering:
Jaargang 18 (2001) nr. 1 pagina's 39-45
Jaar:
2001-01-01
Inhoud:
A survey of zearalenone (ZEA) in corn from various regions of Brazil was carried out by the analysis of 380 corn samples, of which 30 samples (7.8%) were found to be contaminated in the range of 46.7-719 μg/kg. ZEA was extracted with acetonitrile-water (84:16, v/v), cleaned-up on a Romer Mycosep™ 224 column, separated, detected and quanti® ed by high performance liquid chromatography (HPLC). The in-house method characteristics of linearity, accuracy, precision, and detection limit were defined by means of recovery tests with spiked corn samples in the range of 35.8-716 μg/ kg and the analysis of a naturally-contaminated sample (n = 7). The mean recovery for ZEA was 99.4% and the relative standard deviation (RSD) varied from 0.7 to 26.6% in the range studied. The method has been shown to be accurate, quick and reliable for determination of zearalenone in corn.
Tijdschrift beschrijving