Analysing organophosphorus pesticides in wines using graphitized carbon black extraction cartridges
Title:
Analysing organophosphorus pesticides in wines using graphitized carbon black extraction cartridges
Author:
Prieto, Avismelsi
Appeared in:
Food additives and contaminants. Pt. A, Chemistry, analysis, control, exposure & risk assessment
Paging:
Volume 16 (1999) nr. 2 pages 57-61
Year:
1999-01-01
Contents:
A quick and simple method was developed to determine the residues of 15 organophosphorus pesticides of different polarity in grape wine, based on liquid-solid extraction with graphitized carbon black cartridges and capillary gas chromatography. Aliquots of diluted wine (10ml wine 40ml deionized water) and 250mg graphitized carbon black cartridges were used to extract the analytes. The pesticides were eluted with 2 0 . 5ml acetone and 3 2 . 5ml ethyl acetate/acetone (80 : 20). The eluates were then dried with anhydrous sodium sulphate and concentrated to 1ml under a nitrogen current. After adding triphenylphosphate internal standard (i.s.), this extract was analysed by capillary gas chromatography using a nitrogen-phosphorus detector (GC-NPD). Pesticide recovery percentages at levels of 0.01 and 0.02 mug/ml were between 74% and 111% , with a 2-13% relative standard deviation interval. The chromatograms of extracts had few co-extracted species peaks, with no analytical interference. Dilution of the grape wine samples removed most of the pigments, thus producing clear extracts. The results indicate that liquid-solid extraction from wine samples using graphitized carbon is fast (25min per sample), efficient and reproducible for analysing organophosphorus pesticides of different polarity.
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