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| Titel: |
Development of enzyme immunoassay for detection of DDT |
| Auteur: |
Hirano, Masashi Kitamura, Kazuyuki Kato, Ikuo Yanaihara, Chizuko Iwamoto, Ken-Ichi Sekiyama, Makiko Watanabe, Chiho Nakamoto, Takashi Miyamoto, Nobukazu Onishi, Yuta Arizono, Koji |
| Verschenen in: |
Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes |
| Paginering: | Jaargang 43 (2008) nr. 1 pagina's 44-49 |
| Jaar: |
2008-01 |
| Inhoud: |
Dichlorodiphenyltrichloroethane (DDT) is one of the persistent organic pollutants (POPs) widely found in the environment and in the general population. In this study, a direct competitive enzyme immunoassay (EIA) has been developed for the quantitative analysis of DDT. To generate a specific polyclonal antibody for EIA, p, p'-DDT was conjugated to porcine thyroglobulin for rabbit immunization. At optimized EIA conditions, the standard curves ranged from 0.137 to 100 ng/mL with the quantification limit of 0.41 ng/mL. The coefficients of variation (CV%) were 5.42-10.53% for intra-assay and 6.04-7.26% for inter-assay. Cross-reactivities with DDT metabolites (DDTs, including o, p'-DDT, p, p'-DDD, o, p'-DDD, p, p'-DDE, o, p'-DDE, p, p'-dichlorobenzophenone (DCBP), o, p'-DCBP) were investigated. The polyclonal antibody showed relatively low and/or no cross-reactivity with these compounds, and the assay was seen to be highly selective for p, p'-DDT. Moreover, the DDTs could be ranked by their reactivity: DDT > DDD > DDE > DCBP. In addition, the characterization of the polyclonal antibody indicated that the antiserum possesses a high specificity for p, p'-isomers. The results indicated that the developed EIA using this antibody could be a convenient and supplemental analytical tool for monitoring DDT. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 6 van 13 gevonden artikelen
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