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Details van artikel 8 van 9 gevonden artikelen
| Titel: |
Reconstruction of AcMNPV with Helicoverpa hormone receptor 3 and its effect on the Helicoverpa larvae |
| Auteur: |
Shao, Hong-Lian Dong, Du-Juan Hu, Jin-Dong Zhang, Xin-Chang Zhang, Yu-Bao Fu, Qiang Sun, Chen Wang, Jin-Xing Zhao, Xiao-Fan |
| Verschenen in: |
Biocontrol science and technology |
| Paginering: | Jaargang 17 (2007) nr. 1 pagina's 95-104 |
| Jaar: |
2007 |
| Inhoud: |
Helicoverpa hormone receptor 3 (HHR3) is a transcription factor involved in larval molting regulation. To investigate its function and the possibility to improve the insecticidal activity of AcMNPV, HHR3 was expressed in Bac-to-Bac system and an AcMNPV-GFP-HHR3-Polh+ virus was constructed after recovery of the polyhedrin gene. HHR3 expression in Sf21 cells was confirmed by immunofluorescence microscopy. A red color identifying HHR3 was observed using fluorescence microscopy; this confirmed that HHR3 was expressed in Sf21 cells by the introduction of the recombinant virus AcMNPV-GFP-HHR3-Polh+. The AcMNPV-GFP-HHR3-Polh+ infected the hemocytes of H. armigera larvae by hemocoele injection or per oral ingestion. Results showed that AcMNPV-GFP-HHR3-Polh+ resulted in incomplete ecdysis, slower growth and lower median survival time on the Helicoverpa larvae. This recombinant virus may aid in the development of an insecticide that can be used in the field. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 8 van 9 gevonden artikelen
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