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| Titel: |
In vitro study of intracellular IL-1β production and β1 integrins expression in stimulated chondrocytes – Effect of rhein |
| Auteur: |
Gigant-Huselstein, C. Dumas, D. Payan, E. Muller, S. Bensoussan, D. Netter, P. Stoltz, J.F. |
| Verschenen in: |
Biorheology |
| Paginering: | Jaargang 39 (2002) nr. 1-2 pagina's 277-285 |
| Jaar: |
2002-07-11 |
| Inhoud: |
The purpose of the present study was to investigate the intracellular IL-1β production and β1 integrins (α4/β1 and α5/β1) expression on chondrocytes. Chondroytes monolayer (human chondrosarcoma cell line HEM-C55) were incubated for 12, 24 and 48 hours in the presence of Tumor Necrosis Factor-α (TNF-α, Sigma, France) or recombinant human IL-1α (rh-IL1α, Becton Dickinson, France). After direct immunolabelling, cells were either analyzed on FACScan flow cytometer (Becton Dickinson, France), or observed under an epi-fluorescence inverted microscope equipped with the CellScan EPRTM optical scanning acquisition system (IPLab- Scanalytics, USA).We found that the IL-1β mean fluorescence intensity in flow cytometry and in 3D microscopy was increased in the presence of TNF-α or rh-IL-1α, and α4/β1 or α5/β1 expression was higher on stimulated cells than on control cells. On the other hand, we have evaluated the in vitro effects of rhein (10-5 M, Negma, France), an active metabolite of diacerein, on the intracellular IL-1β and β1 integrins expressed by stimulated or no-stimulated chondrocytes. The results indicated that rhein leads to a reduction of IL-1β synthesis whereas a weak decrease of β1 integrins receptors expression is observed. From this study, it seems that rhein partially reduce cytokine-induced intracellular IL-1β production, and it has a weak action on α4/β1 or α5/β1 receptors. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 16 van 31 gevonden artikelen
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