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Details for article 4 of 8 found articles
| Title: |
Immunological Evidence for Peptide-Carbohydrate Mimicry with a Group A Streptococcus Polysaccharide-Mimetic Peptide |
| Author: |
Silvia Borrelli Rehana B. Hossany Susan Findlay B. Mario Pinto |
| Appeared in: |
American journal of immunology |
| Paging: | Volume 2 (2006) nr. 4 pages 77-87 |
| Year: |
2006 |
| Contents: |
The immunogenicity of a peptide-protein conjugate developed by linking a peptide mimicDRPVPY of the Group A Streptococcus cell-wall polysaccharide (GAS-CWPS), to tetanus toxoid(TT) was examined. BALB/c mice were immunized three times subcutaneously following homologousor heterologous prime/boost strategies at 4- or 6- week intervals in two different experiments.DRPVPY-TT, CWPS-TT, heat-killed, pepsin-treated GAS bacteria (with exposed polysaccharide) andTT, were used as immunogens with alum as adjuvant. Antibody titers were determined by ELISA withGAS bacteria (with exposed polysaccharide) and DRPVPY-linked to bovine serum albumin (BSA,DRPVPY-BSA) as solid phase antigens. All mice primed with DRPVPY-TT developed high IgG antipeptideand anti-GAS titers. The binding of polyclonal anti-peptide antibodies to GAS could beinhibited by purified CWPS, synthetic oligosaccharides corresponding to CWPS, DRPVPY-BSA,DRPVPY and DRPVP, as assessed by competitive-inhibition ELISA. Anti-oligosaccharide titers werealso obtained upon titration of anti-peptide sera with synthetic oligosaccharide-BSA conjugates. Allmice primed with CWPS-TT and mice primed and boosted with GAS developed IgG anti-peptidetiters. These data demonstrate conclusively the cross-reactivity of the immune responses and supportthe hypothesis of antigenic mimicry of the GAS-CWPS by the hexapeptide DRPVPY. However, miceboosted with DRPVPY-TT, after 6-8 weeks, showed a decrease in IgG anti-GAS titers, but an increasein IgG anti-peptide titers, suggesting carrier-induced suppression of the response to polysaccharide.Strategies are outlined for further refinement of a DRPVPY conjugate as a surrogate of the cell-wallpolysaccharide for use in vaccines against Group A Streptococcus. |
| Publisher: |
Science Publications (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 4 of 8 found articles
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