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Details for article 3 of 8 found articles
| Title: |
Foxp3-Mediated Immunity of Human Pancreatic Cancer Cell Line PANC-1 |
| Author: |
Renxi Wang Gencheng Han Jianan Wang Guojiang Chen Ruonan Xu Liyan Wang Xia Li Beifen Shen Yan Li |
| Appeared in: |
American journal of immunology |
| Paging: | Volume 5 (2009) nr. 4 pages 101-107 |
| Year: |
2009 |
| Contents: |
Problem statement: More and more evidences have shown that human cancer cells can express Foxp3, the regulatory T cell-associated transcription factor. The role of Foxp3 in human cancer cells is still unclear. We detected Foxp3 expression in human pancreatic cancer cell line PANC-1. SiRNA assays showed that Foxp3 expression suppressed the expression of TGF-β and IL-10 which could induce immune tolerance. Approach: Thus, we proposed that Foxp3 expression in PANC-1 cells controlled the response but not tolerance characteristics of the cells. To prove the proposal, we first developed the PANC-1 cell-induced the immune response model. PANC-1 cells induced response in autoimmune Non-Obese Diabetes (NOD) mice aged of >12 weeks. In vitro cell co-cultured assay demonstrated that PANC-1 induced response in splenocytes from NOD mice aged of >12 weeks. Results: In the same time, we found that inflammatory cytokine such as INF-γ, IL-2 and IL-17 increased and anti-inflammatory cytokine such as IL-4, IL-10 and TGF-β decreased in the co-cultured supernatant. These results demonstrate that PANC-1 cells induced response in splenocytes from NOD mice aged of >12 weeks. SiRNA assays showed that Foxp3 in PANC-1 cells controlled the response in the co-cultured cells. Conclusion: The results suggested that Foxp3 control the immunological response characteristics of PANC-1 cells in autoimmune condition. |
| Publisher: |
Science Publications (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 3 of 8 found articles
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