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                             196 results found
no title author magazine year volume issue page(s) type
1 Activity-dependent labeling and manipulation of fentanyl-recruited striatal ensembles using ArcTRAP approach Xie, Xueyi

4 3 p.
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2 A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cells Li, Xisheng

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3 Adverse outcome pathway-based analysis of liver steatosis in vitro using human liver cell lines Karaca, Mawien

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4 A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry Engevik, Kristen A.

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5 A histological protocol for quantifying the birthrates of specific subtypes of olfactory sensory neurons in mice Hossain, Kawsar

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6 A knowledge-integrated deep learning framework for cellular image analysis in parasite microbiology Feng, Ruijun

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7 A light-driven selective protocol for on-demand methanol and formic acid syntheses with a recyclable GaN catalyst Han, Jing-Tan

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8 Analysis of autophagy deficiency and cytotoxicity in autophagy-deficient human embryonic stem cell-derived neurons Korsgen, Miriam E.

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9 Analysis of polar primary metabolites in biological samples using targeted metabolomics and LC-MS Turtoi, Evgenia

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10 Analyzing the topology of N-linked glycans by PNGase F accessibility assay Wang, Jingcheng

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11 An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments Zeng, Xiaoqian

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12 Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics Barnes V, Lucien

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13 A protocol for applying low-coverage whole-genome sequencing data in structural variation studies Liu, Qi

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14 A protocol for gradient coating design zinc anodes for stable zinc ion batteries Chen, Wenjun

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15 A protocol for high-throughput screening for immunomodulatory compounds using human primary cells Chew, Katherine

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16 A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons Nishimura, Kaneyasu

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17 A protocol for the use of cloud-based quantum computers for logical network analysis of biological systems Weidner, Felix M.

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18 A protocol for visualization of murine in situ neurovascular interfaces Dang, Danielle D.

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19 A protocol for visualizing active cathepsin K in osteoclasts with a quenched-fluorescence-activity-based probe Korba, Aleksandra

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20 A protocol to analyze single-cell RNA-seq data from Mycobacterium tuberculosis-infected mice lung Akter, Sadia

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21 A protocol to establish cell line models from rare pediatric solid tumors Chew, Nicole

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22 A protocol to investigate neural coupling of brain oscillations in rodents using in vivo electrophysiological recordings Wang, Cong

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23 ASSURED-optimized CRISPR protocol for knockout/SNP knockin in hiPSCs Ludwik, Katarzyna A.

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24 A stereoselective protocol for the synthesis of a molecular cinquefoil (51) knot Han, Jing

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25 ATAC-seq protocol for the profiling of chromatin accessibility of in situ fixed quiescent and activated muscle stem cells Dong, Anqi

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26 Attachment culture of cortical organoids at the microwell air-liquid interface Tang, Jiyuan

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27 Automated qualitative batch measurement of lipid droplets in the liver of bird using ImageJ Nishad, Anurag

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28 Breast cancer PDxO cultures for drug discovery and functional precision oncology Scherer, Sandra D.

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29 Calculating RNA degradation rates using large-scale normalization in mouse embryonic stem cells Viegas, Juliane Oliveira

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30 Cell-surface biotinylation of GABAA receptors in mouse hippocampal slices after sevoflurane anesthesia Ariza, Anthony

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31 Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry Duan, Jinzhi

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32 Characterizing interaction between the juxtamembrane region of the single transmembrane protein and membrane using chemically synthesized peptides Sato, Takeshi

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33 Computational workflow for investigating highly variable genes in single-cell RNA-seq across multiple time points and cell types Arora, Jantarika Kumar

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34 CRIC-seq protocol for in situ profiling of proximal RNA-RNA contacts associated with RNA-binding proteins Ye, Rong

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35 CRISPR generation of CSF1R-G795A human microglia for robust microglia replacement in a chimeric mouse model Chadarevian, Jean Paul

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36 3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells Govindasamy, Niraimathi

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37 Deriving a continuum score for group 3 and 4 medulloblastoma tumor samples analyzed via RNA-sequencing or DNA methylation microarray Hacking, James P.R.

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38 Detection of membrane-anchoring lipid modifications of proteins in cells by radioactive metabolic labeling Bhat, Sameer Ahmed

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39 Detection of viable commensal bacteria in murine melanoma tumors by culturomics Phelps, Catherine M.

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40 Developmental-stage specific single-cell human embryo dissociation Lammers, Jenna

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41 Differential affinity purification and mass spectrometry analysis of two nuclear pore complex isoforms in yeast S. cerevisiae Bensidoun, Pierre

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42 Differential systemic decellularization in vivo to study molecular changes in each vasculature layer in murine models of disease Gallart-Palau, Xavier

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43 Electrochemical water splitting by a bidirectional electrocatalyst Aziz, S.K. Tarik

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44 Engineering the sialome of mammalian cells with sialic acid mimetics Hornikx, Daniël L.A.H.

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45 Enrichment of carcinogen-driven “mitochondria-primed” human skin stem cells and their identification using single-cell analyses Agarwala, Swati

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46 Enzyme-free isolation of mesenchymal stem cells from decidua basalis of the human placenta Dutta Gupta, Srishti

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47 Enzyme-linked immunosorbent assay to detect surface marker proteins of extracellular vesicles purified from human urine Takizawa, Keiichi

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48 Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures Fietz, Agnes

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49 exo-FISH: Protocol for detecting DNA breaks in repetitive regions of mammalian genomes Saayman, Xanita

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50 FISH-Flow to quantify nascent and mature ribosomal RNA in mouse and human cells Antony, Charles

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51 Flow cytometric analysis of phosphatidylcholine metabolism using organelle-selective click labeling Tsuchiya, Masaki

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52 Fluorescence-microscopy-based assay assessing regulatory mechanisms of global genome nucleotide excision repair in cultured cells Kusakabe, Masayuki

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53 Functional in vitro and in vivo analysis of biosynthetic genes by heterologous expression in E. coli Kramer, Jil-Christine

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54 Generation of epitope tag knock-in mice with CRISPR-Cas9 to study the function of endogenous proteins Zhang, Zhao

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55 Generation of gene-of-interest double allele knockout clones in primary human T cells by CRISPR Wu, Ling

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56 Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells Ciarpella, Francesca

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57 Generation of vascularized human cardiac organoids for 3D in vitro modeling Voges, Holly K.

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58 Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells Mazuecos, Lorena

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59 Glyoxal-based fixation of Drosophila embryos for immunofluorescence staining and RNA in situ hybridization Amin, Shrunali

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60 Histological analysis of invasive glioblastoma organoids embedded in a 3D collagen matrix Guyon, Joris

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61 Hormone immunolabeling in resin-embedded Arabidopsis tissues Herrera-Ubaldo, Humberto

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62 Imaging and quantitative analysis of integrin-dependent cell-matrix adhesions van Stalborch, Anne-Marieke D.

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63 Immunofluorescence protocol for localizing protein targets in brain tissue from diverse model and non-model mammals Ciani, Caterina

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64 Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates Yu, Gang

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65 Intracellular ATP delivery to in vitro expanded mouse CD27− γδ T cells Wang, Yunke

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66 Isolation and detection of extracellular vesicles from melanoma cells and liquid biopsies using size-exclusion chromatography and nano-flow cytometry Lattmann, Evelyn

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67 Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics Zhang, Junyi

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68 Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers Liu, Xiao

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69 Kidney capsule transplantation of chemically treated embryonic murine tooth germs Jimenez-Rojo, Lucia

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70 Large-scale single-cell cloning of genome-edited cultured human cells by On-chip SPiS Takahashi, Gou

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71 Mapping alternative polyadenylation in human cells using direct RNA sequencing technology Polenkowski, Mareike

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72 Mapping neuronal ensembles and pattern-completion neurons through graphical models O’Neil, Darik A.

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73 Master regulator activity QTL protocol to implicate regulatory pathways potentially mediating GWAS signals using eQTL data Hoskins, Jason W.

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74 Measuring endogenous levels of unconjugated bilirubin released from isolated murine brain tissue during oxygen-glucose deprivation Liu, Han-Wei

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75 Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq De Cauwer, Aurore

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76 Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension Tsurugizawa, Tomokazu

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77 Modified Capillary Feeder assay using micro-tips to measure real-time feeding in Drosophila Segu, Aishwarya

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78 Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice Di Gesù, Claudia M.

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79 Mouse intraductal modeling of primary ductal carcinoma in situ Hutten, Stefan J.

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80 Multiplexed targeted analysis of polyunsaturated fatty acids and oxylipins using liquid chromatography-tandem mass spectrometry Turtoi, Evgenia

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81 Non-negative tensor factorization workflow for time series biomedical data Tsuyuzaki, Koki

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82 On-bead purification and nanodisc reconstitution of human chemokine receptor complexes for structural and biophysical studies Gu, Siyi

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83 Optimized protocol for mouse footpad immune cell isolation for single-cell RNA sequencing and flow cytometry Hameed, Muddassar

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84 Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons Sheta, Razan

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85 Optimized single-cell RNA sequencing protocol to study early genome activation in mammalian preimplantation development Boskovic, Nina

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86 Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells Matsuzaki, Takahisa

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87 Profiling and verifying the substrates of E3 ubiquitin ligase Rsp5 in yeast cells Fang, Shuai

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88 Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture Semper, Cameron

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89 Protocol for a distributed smart building solution using semi-physical simulation Yan, Hu

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90 Protocol for ambient CO2 capture and conversion into HCOOH and NH4H2PO4 Jiang, Shuchao

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91 Protocol for analyzing antibody responses to glycoprotein antigens using electron-microscopy-based polyclonal epitope mapping Turner, Hannah L.

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92 Protocol for automated multivariate quantitative-image-based cytometry analysis by fluorescence microscopy of asynchronous adherent cells Besse, Laetitia

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93 Protocol for bulk and single-nuclei chromatin accessibility quantification in mouse liver tissue Korenfeld, Noga

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94 Protocol for calcium imaging of dorsal and ventral CA1 neurons in head-fixed mice Yun, Miru

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95 Protocol for constructing a hierarchical host-guest supramolecular self-assembly system in water Chen, Xiao

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96 Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection Li, Kun

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97 Protocol for culturing the endocrine junctional zone of the mouse placenta in serum-free medium Yung, Hong Wa

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98 Protocol for deep proteomic profiling of formalin-fixed paraffin-embedded specimens using a spectral library-free approach Soni, Rajesh Kumar

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99 Protocol for density gradient neutrophil isolation and flow cytometry-based characterization from human peripheral blood Kuang, Yi

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100 Protocol for deriving proximity, affinity, and stoichiometry of protein interactions using image-based quantitative two-hybrid FRET Feldmann, Colin

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101 Protocol for designing and bioprinting multi-layered constructs to reconstruct an endothelial-epithelial 3D model Backes, Eduardo Henrique

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102 Protocol for detection of ferroptosis in cultured cells Murray, Magdalena B.

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103 Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe Jin, Ji Hye

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104 Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cells Chowdhury, Shanin

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105 Protocol for establishing a coculture with fibroblasts and colorectal cancer organoids Wallisch, Svenja

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106 Protocol for estimating the impact of climate change on economic growth and inequality under climate policies Tang, Yun

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107 Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model Lee, Yu Jin

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108 Protocol for expression of murine milk using modified human breast pump parts Meyer, Cydney

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109 Protocol for extracting live blastoderm cells from embryos of annual killifish Vásquez-Sepúlveda, Sebastian

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110 Protocol for fabricating pseudocapacitor electrodes using ultra-short laser pulses for in situ nanostructure generation Khosravinia, Kavian

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111 Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID Zhu, Jingtan

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112 Protocol for generating airway organoids from 2D air liquid interface-differentiated nasal epithelia for use in a functional CFTR assay Rodenburg, Lisa W.

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113 Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell-derived cerebral organoids Feng, Yilin

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114 Protocol for generating monoclonal CRISPR-Cas9-mediated knockout cell lines using RNPs and lipofection in HNSCC cells Geyer, Fabian

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115 Protocol for generating reproducible miniaturized controlled midbrain organoids Chen, Muwan

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116 Protocol for geometric transformation of cognitive maps for generalization across hippocampal-prefrontal circuits Shin, Justin D.

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117 Protocol for high-throughput electron tomography exemplified on centrioles in primary human plasma cells Köhrer, Sebastian

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118 Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling Koenig, Claire

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119 Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair Jgamadze, Dennis

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120 Protocol for identifying immune checkpoint on circulating tumor cells of human pancreatic ductal adenocarcinoma by single-cell RNA sequencing Liu, Xiaowei

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121 Protocol for imaging nuclear pore complexes on isolated nuclei of zebrafish early embryos by field emission scanning electron microscopy Shen, Weimin

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122 Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids Ober, Kimberley

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123 Protocol for in vitro assessment of human monocyte transendothelial migration using a high-throughput live cell imaging system Ladaigue, Ségolène

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124 Protocol for in vivo and in vitro activation of NLRP3 inflammasome in mice using monosodium urate Liu, Didi

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125 Protocol for isolating human BCAM-positive corneal progenitor cells by flow cytometry and cell sorting Sasamoto, Yuzuru

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126 Protocol for isolation of mouse pre-implantation embryos for gene expression analysis Varghese, Pallavi Chinnu

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127 Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes Ichikawa, Takehiko

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128 Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay Spencer-Smith, Russell

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129 Protocol for measuring visual preferences of freely swimming Xenopus laevis tadpoles Udoh, Uwemedimo G.

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130 Protocol for mechanochemistry-guided assembly strategy for enzyme encapsulation using covalent organic frameworks Gao, Rui

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131 Protocol for modulating the noradrenergic pathway from locus coeruleus to heart to prevent sudden unexpected death in epilepsy in mouse models Xu, Qing

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132 Protocol for neonatal respiratory syncytial virus infection in mice and immune analysis of infected lungs and bronchoalveolar lavage fluid Adhikari, Sandeep

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133 Protocol for observing microtubules and microtubule ends in both fixed and live primary microglia cells Sanchini, Caterina

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134 Protocol for optimizing production and quality control of infective EcoHIV virions Alfar, Hammodah R.

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135 Protocol for predicting peptides with anticancer and antimicrobial properties by a tri-fusion neural network Han, Jiyun

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136 Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines Lyu, Yonglei

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137 Protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms Li, Yibo

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138 Protocol for profiling in vitro intratumor heterogeneity using spatially annotated single-cell sequencing Smit, Myrthe M.

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139 Protocol for purification and enzymatic characterization of members of the human macrophage migration inhibitory factor superfamily Parkins, Andrew

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140 Protocol for purification of cells in their native state using reversible aptamer-antidote pairs Requena, Martin D.

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141 Protocol for quantitative analysis of RNA 3′-end processing induced by disassociated subunits using chromatin-associated RNA-seq data Huang, Jie

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142 Protocol for refining metagenomic binning with BinSPreader Ochkalova, Sofia

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143 Protocol for simulating foraging behavior strategy and dual-choice predatory sensory cue experiments in aerial-hawking bats Gong, Lixin

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144 Protocol for siRNA-mediated TET1 knockdown during differentiation of human embryonic stem cells into definitive endoderm cells Liufu, Zhongqi

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145 Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro Goncheva, Mariya I.

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146 Protocol for the automatic extraction of epidemiological information via a pre-trained language model Wang, Zhizheng

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147 Protocol for the isolation and purification of human follicular dendritic cells for functional assays Breeuwsma, Martijn

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148 Protocol for the isolation of mouse senescence-associated CD4+ T cells using flow cytometry and functional assays Fukushima, Yuji

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149 Protocol for the single-crystal growth of chromium-based RECrO3 compounds Fu, Ying Ellie

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150 Protocols for transgenesis at a safe harbor site in the Xenopus laevis genome using CRISPR-Cas9 Shibata, Yuki

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151 Protocol to analyze endogenous translesion DNA synthesis in single mammalian cells Egger, Tom

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152 Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays Tse, Samantha Y.

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153 Protocol to characterize target components of Gelsemium using an in-house herb database and high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry Zuo, Meng-Ting

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154 Protocol to conduct functional magnetic resonance spectroscopy in different age groups of human participants Frank, Sebastian M.

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155 Protocol to dissociate and isolate wide-diversity single cells by density gradient centrifugation from human hepatoblastoma tissue Zhou, Xianchao

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156 Protocol to establish an oviduct epithelial cell line derived from Gallus gallus using Percoll for in vitro validation of recombinant proteins Loubser, Chiron

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157 Protocol to expand and CRISPR-Cas9 genomic edit murine MAIT cells for subsequent in vivo studies du Halgouet, Anastasia

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158 Protocol to generate and utilize pancreatic tissue slices to study endocrine and exocrine physiology in situ from mouse and human tissue Panzer, Julia K.

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159 Protocol to generate fast-dissociating recombinant antibody fragments for multiplexed super-resolution microscopy Zhang, Qianli

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160 Protocol to generate large human intestinal organoids using a rotating bioreactor Takahashi, Junichi

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161 Protocol to identify amino acids bound to tRNA by aminoacylation using mass spectrometry Finkler, Marc

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162 Protocol to identify human subcellular alternative protein interactions using cross-linking mass spectrometry Garcia-del Rio, Diego Fernando

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163 Protocol to identify multiple protein targets and therapeutic compounds using an in silico polypharmacological approach Simha N, Akshaya

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164 Protocol to image and quantify nuclear pore complexes using high-resolution laser scanning confocal microscopy Mich-Basso, Jocelyn D.

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165 Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses Chen, Hao

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166 Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging Zilliox, Marie

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167 Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seq Vélez-Bermúdez, Isabel Cristina

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168 Protocol to minimize the confounding effect of cold stress on socially isolated mice using thermoneutral housing Appana, Bhavya

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169 Protocol to optimize the biobanking of ovarian cancer organoids by accommodating patient-specific differences in stemness potential Trillsch, Fabian

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170 Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum Blöbaum, Luisa

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171 Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates Stanis, Noah

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172 Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique Ji, Ya-Wei

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173 Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets Seigneurin-Berny, Daphné

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174 Protocol to track the biosynthesis of cholesterol in cultured HCC cells using 13C compound-specific stable isotopic tracers Cybulski, Jonathan D.

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175 Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain Mino, Takeharu

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176 Quantification of membrane-bound cytokine receptors by calibrated flow cytometry Miri, Niloufarsadat

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177 Quantification of total and phosphorylated STAT3 by calibrated western blotting Köhler, Nadine

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178 Quantifying mitochondrial redox and bilirubin content in intact primary hepatocytes of obese mice using fluorescent reporters Belmas, Thomas

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179 Quantitative cross-linking via engineered cysteines to study inter-domain interactions in bacterial collagenases Serwanja, Jamil

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180 Quantitative cytofluorimetric analysis of mouse neutrophil extracellular traps Poli, Valentina

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181 RNA extraction protocol from low-biomass bacterial Nitrosomonas europaea and Nitrobacter winogradskyi cultures for whole transcriptome studies Verbeelen, Tom

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182 Semi-automated colony-forming unit counting for biosafety level 3 laboratories Janakiraman, Soorya

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183 Single-cell RNA sequencing and analysis of rodent blood stage Plasmodium Jentho, Elisa

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184 Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila Pérez-Mojica, J. Eduardo

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185 Soft agar colony formation assay to quantify mouse embryonic fibroblast transformation after Salmonella infection Stévenin, Virginie

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186 Studying naturalistic human communication using dual-EEG and audio-visual recordings Mazzini, Sara

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187 Synthesis and characterization of highly diluted polyanionic iron(II) spin crossover systems Moneo-Corcuera, Andrea

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188 Synthesis of fluorinated triphenylphosphonium analogs that improve cancer cell selectivity and in vivo detection Keyes, Robert F.

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189 Synthesis of gold–tin alloy nanoparticles with tunable plasmonic properties Branco, Anthony J.

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190 Three methods for examining the de novo proteome of microglia using BONCAT bioorthogonal labeling and FUNCAT click chemistry Carlisle, Alison Keolani

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191 Tracking the growth fate of single cells and isolating slow-growing cells in human colorectal cancer organoids Coppo, Roberto

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192 Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris Yamamoto, Wataru

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193 Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations Merenciano, Miriam

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194 Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset Bunning, Angela R.

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195 Using the single-cell imaging system and orthotropic footpad injection to establish mouse models for experimental and spontaneous melanoma metastasis Yu, Yanlin

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196 Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation Ferriz, Margarita

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                             196 results found
 
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