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Details van artikel 6 van 12 gevonden artikelen
| Titel: |
Improved transcriptome quantification and reconstruction from RNA-Seq reads using partial annotations |
| Auteur: |
Mangul, Serghei Caciula, Adrian Glebova, Olga Mandoiu, Ion Zelikovsky, Alex |
| Verschenen in: |
In silico biology |
| Paginering: | Jaargang 11 (2012) nr. 5-6 pagina's 251-261 |
| Jaar: |
2012-11-30 |
| Inhoud: |
The paper addresses the problem of how to use RNA-Seq data for transcriptome reconstruction and quantification, as well as novel transcript discovery in partially annotated genomes. We present a novel annotation-guided general framework for transcriptome discovery, reconstruction and quantification in partially annotated genomes and compare it with existing annotation-guided and genome-guided transcriptome assembly methods. Our method, referred as Discovery and Reconstruction of Unannotated Transcripts (DRUT), can be used to enhance existing transcriptome assemblers, such as Cufflinks [3], as well as to accurately estimate the transcript frequencies. Empirical analysis on synthetic datasets confirms that Cufflinks enhanced by DRUT has superior quality of reconstruction and frequency estimation of transcripts. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 6 van 12 gevonden artikelen
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