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| Titel: |
Expression of recombinant human anti-MAG antibodies in non-lymphoid mammalian cells |
| Auteur: |
Spatz, Linda A. Daugherty, Bruce L. DeMartino, Julie A. Mark, George E. Latov, Norman |
| Verschenen in: |
Human antibodies |
| Paginering: | Jaargang 3 (2013) nr. 2 pagina's 107-111 |
| Jaar: |
2013-01-14 |
| Inhoud: |
The variable heavy and light chain genes of a monoclonal, IgM, anti-MAG antibody from a patient with neuropathy were inserted into expression vectors containing the gamma and kappa constant regions respectively and co-transfected into monkey kidney CVIP cells. The expressed antibody had the same antigenic specificity but significantly lower avidity than the native IgM, anti-MAG, antibody as detected by ELISA. When the variable heavy chain gene of the anti-MAG antibody was co-transfected with the variable light chain gene from another monoclonal, IgM, anti-MAG antibody, a fully assembled antibody was expressed as determined by a trapping ELISA, but it did not bind to (MAG) or to sulfated glucuronic acid paragloboside, indicating that both heavy and light chains contribute to the binding activity. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 7 gevonden artikelen
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