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Details van artikel 4 van 7 gevonden artikelen
| Titel: |
Generation of hybrid hybridomas secreting human IgM class hybrid antiricin and antidiphtheria toxin antibodies |
| Auteur: |
Shinmoto, Hiroshi Dosako, Shun'ichi Tachibana, Hirofumi Yamada, Koji Shirahata, Sanetaka Murakami, Hiroki |
| Verschenen in: |
Human antibodies |
| Paginering: | Jaargang 2 (2013) nr. 1 pagina's 39-41 |
| Jaar: |
2013-01-14 |
| Inhoud: |
Two lines of mouse-human hybridomas were fused to produce human bifunctional antibodies. A hybridoma that secretes human IgM (κ) class antiricin monoclonal antibody was treated with actinomycin D to inhibit cell growth. Another cell line, secreting human IgM (λ) class antidiphtheria toxin monoclonal antibody, was 6-thioguanine resistant (HAT sensitive). Six hybridomas secreting IgM with both κ and λ light chains were obtained. Two of them secreted antibodies with antiricin specificity (κ and λ light chains) and antidiphtheria toxin specificity (κ and λ light chains). Antidiphtheria toxin antibodies secreted from other hybrid hybridomas did not contain κ light chain, but λ chain. A hybrid hybridoma clone 5–29 secreted complete hybrid IgM with lowered affinity to diphtheria toxin. These results suggested unbalanced recombination of immunoglobulin polypeptide chains in hybrid hybridomas. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 7 gevonden artikelen
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