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Details for article 59 of 125 found articles
| Title: |
Human κ chain expression in a λ phage vector: methods of isolating amplified cDNA affects cloning efficiency |
| Author: |
Lazaridis, Konstantinos Dang, Howard Talal, Norman |
| Appeared in: |
Human antibodies |
| Paging: | Volume 4 (2013) nr. 3 pages 104-106 |
| Year: |
2013-01-14 |
| Contents: |
Three common methods of isolating amplified DNA were evaluated for their effects on cloning efficiency in a phage vector designed to express human κ chain. The “glass milk” technique gave higher cloning efficiency and protein expression than phenol-chloroform extraction or microfiltration. This shows that the quality of amplified cDNA should be considered when studying the human antibody repertoire using this vector system. |
| Publisher: |
IOS Press |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 59 of 125 found articles
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