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Details van artikel 47 van 132 gevonden artikelen
| Titel: |
Phage surface presentation and secretion of antibody fragments using an adaptable phagemid vector |
| Auteur: |
Lah, Maria Goldstraw, Alanna White, Jacinta F. Dolezal, Olan Malby, Robyn Hudson, Peter J. |
| Verschenen in: |
Human antibodies |
| Paginering: | Jaargang 5 (2013) nr. 1-2 pagina's 48-56 |
| Jaar: |
2013-01-14 |
| Inhoud: |
Phagemid vectors have been developed which promise to supersede hybridoma technology for the selection and production of human antibodies. We have modified an existing phagemid vector to improve the stability of synthesized soluble antibody fragments. The vector allows the antibody fragment to be produced: i) as a soluble protein incorporating a stable carboxyl terminal octapeptide (FLAG) or, ii) on the surface of a bacteriophage fused to a minor coat protein (the gene III protein). The antibody gene encoding the well characterized monoclonal antibody NC10 (an antibody that recognizes the neuraminidase of the influenza strain N9) was inserted as a single chain Fν construct into the phagemid vectors pHFA and pHFA/SacII. Western blotting, ELISA and electron microscopy studies showed that recombinant clones could be manipulated to either synthesize soluble protein into the peri plasm or present the protein on the surface of bacteriophage. Cosynthesis of GroEL and GroES chaperon ins resulted in complete proteolysis of the scFνNC10-FLAG-gIIIp fusion product and did not improve total phage production. Coexpression of chaperonins should be used with caution for library construction due to the expected selection pressure for protease resistant gene III fusions. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 47 van 132 gevonden artikelen
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