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| Titel: |
A homogeneous HLA-B*27 genotyping assay using dried reagent mixtures |
| Auteur: |
Kiviniemi, Minna Ilonen, Jorma Lövgren, Timo |
| Verschenen in: |
Disease markers |
| Paginering: | Jaargang 27 (2009) nr. 2 pagina's 85-91 |
| Jaar: |
2009-11-05 |
| Inhoud: |
The presence of HLA-B*27 allele with patients suspected with ankylosing spondylitis can be used in the diagnostic process. We have developed an assay for typing for the HLA-B*27 in whole blood dried on sample collection cards using pre-dried reagent wells and homogeneous time-resolved fluorescence based PCR approach. Essentially only the sample needs to be added to the dry ready-to-use reaction well in order to start the homogenous amplification assay. The method was validated with 229 samples also typed with an existing DELFIA-based method and results of both assays were 100% concordant. The dried reagents were shown to be stable at least up to eight weeks at room temperature without any decline in their performance. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 1 van 7 gevonden artikelen
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