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| Titel: |
Identification by differential display PCR of the selenoprotein thioredoxin reductase as a 1\alpha,25(OH)_2-vitamin D_3-responsive gene in human osteoblasts – regulation by selenite |
| Auteur: |
N. Schütze M. Bachthaler A. Lechner J. Köhrle F. Jakob |
| Verschenen in: |
BioFactors |
| Paginering: | Jaargang 7 (2001) nr. 4 pagina's 299-310 |
| Jaar: |
2001-04-01 |
| Inhoud: |
1\alpha,25(OH)_{2} vitamin D_{3} (1,25(OH)_{2}D_{3}) is a potent hormone, stimulating bone cell growth and differentiation. In order to detect novel targets for 1,25(OH)_{2}D_{3} action, we applied differential display PCR (ddPCR) to human fetal osteoblasts (FOB cells). By ddPCR analysis, we identified the selenoprotein thioredoxin reductase (TRR) as a 1,25(OH)_{2}D_{3}-responsive gene. In FOB cells, the response of TRR mRNA steady state levels to 1,25(OH)_{2}D_{3} was fast and transient. Maximal stimulation was observed after one hour of 1,25(OH)_{2}D_{3} treatment, thereafter TRR steady state mRNA levels declined to control levels. This transient response of TRR mRNA was not reflected at the TRR enzyme activity level upon treatment with 1,25(OH)_{2}D_{3} for up to 48 h. Sodium selenite added to differentiated FOB cells increased TRR enzyme activity 2.6-fold, whereas no selenite effect on TRR mRNA steady state levels was measurable. Our data might provide a link between the induction of a differentiation program by 1,25(OH)_{2}D_{3} and the expression of the selenium responsive TRR system in human osteoblasts. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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