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| Titel: |
Enzymology of the fermentation of acetate to methane by Methanosarcina thermophila |
| Auteur: |
James G. Ferry |
| Verschenen in: |
BioFactors |
| Paginering: | Jaargang 6 (2001) nr. 1 pagina's 25-35 |
| Jaar: |
2001-04-01 |
| Inhoud: |
Biologically-produced CH<inf>4</inf> derives from either the reduction of CO<inf>2</inf> or the methyl group of acetate by two separate pathways present in anaerobic microbes from the Archaea domain. Elucidation of the pathway for CO<inf>2</inf> reduction to CH<inf>4</inf>, the first to be investigated, has yielded several novel enzymes and cofactors. Most of the CH<inf>4</inf> produced in nature derives from the methyl group of acetate. Methanosarcina thermophila is a moderate thermophile which ferments acetate by reducing the methyl group to CH<inf>4</inf> with electrons derived from oxidation of the carbonyl group to CO<inf>2</inf>. The pathway in M. thermophila is now understood on a biochemical and genetic level comparable to understanding of the CO<inf>2</inf>-reducing pathway. Enzymes have been purified and characterized. The genes encoding these enzymes have been cloned, sequenced, transcriptionally mapped, and their regulation defined on a molecular level. This review emphasizes recent developments concerning the enzymes which are unique to the acetate fermentation pathway in M. thermophila. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 8 gevonden artikelen
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