The characterization and molecular structure of hepatoproliferin: A liver regeneration factor from rat hepatocytes
Titel:
The characterization and molecular structure of hepatoproliferin: A liver regeneration factor from rat hepatocytes
Auteur:
Oosthuizen, Mathys M.J. Lambrechts, Hugo
Verschenen in:
BioFactors
Paginering:
Jaargang 30 (2008) nr. 1 pagina's 49-65
Jaar:
2008-01-07
Inhoud:
Hepatoproliferin (HPF) was purified from regenerating rat livers as an oligomeric entity (big-HPF) from which the monomeric form (small-HPF) could be obtained using disaggregating conditions. By using a solid-phase ion-exchange method, small-HPF was forced to dissociate into two charged ionic species, namely norepinephrine (NE) and a sulfonated disaccharide with a molecular structure consisting of D-glucuronic acid bound to glucosamine 2,6-disulfate by a β-glycosidic linkage having a β, 1 → 4 configuration. Monomeric HPF stemmed from the formation of three electrostatic bonds between the protonated amine groups of three norepinephrines, of which two bind to the deprotonated sulfonic groups of glucosamine 2,6-disulfate and one to the deprotonated carboxylic group of glucuronic acid, to constitute a tightly associated complex with a molecular mass of 1046 Da. This represents one of the two purified isoforms of small-HPF. The other isoform, which has a lower molecular mass of 877 Da, lack one NE, leaving the weaker carboxylic group of glucuronic acid unoccupied, to constitute a more acidic form of HPF.
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