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Details van artikel 9 van 32 gevonden artikelen
| Titel: |
Coenzyme Q and the regulation of intracellular steady-state levels of superoxide in HL-60 cells |
| Auteur: |
González-Aragón, David Burón, María I. López-Lluch, Guillermo Hermán, María D. Gómez-Díaz, Consuelo Navas, Plácido Villalba, José M. |
| Verschenen in: |
BioFactors |
| Paginering: | Jaargang 25 (2006) nr. 1-4 pagina's 31-41 |
| Jaar: |
2006-07-17 |
| Inhoud: |
The present work was set to study how CoQ concentrations affected steady-state levels of superoxide in a cellular model of partial CoQ_{10} deficiency in cultured human myeloid leukemia HL-60 cells. Culturing HL-60 cells in the presence of p-aminobenzoate, a competitive inhibitor of polyprenyl-4-hydroxybenzoate transferase (Coq2p), produced a significant decrease of CoQ_{10} levels without affecting cell viability. Concomitant decreases in CoQ-dependent electron transport activity and mitochondrial membrane potential were observed under these conditions. Intracellular superoxide was significantly elevated in cells treated with p-aminobenzoate, both under serum-containing and serum-free conditions, and this effect was reversed by exogenous CoQ_{10}. A slight increase of superoxide was also observed in CoQ_{10}-supplemented cells in the absence of serum. Our results support a requirement for CoQ_{10} to control superoxide levels in HL-60 cells. The importance of extramitochondrial sources of superoxide in cells with impaired CoQ_{10} biosynthesis is discussed. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 9 van 32 gevonden artikelen
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