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| Titel: |
Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria |
| Auteur: |
Emil Rudolf Kamil Rudolf Miroslav Cervinka |
| Verschenen in: |
BioFactors |
| Paginering: | Jaargang 23 (2005) nr. 2 pagina's 107-120 |
| Jaar: |
2005-09-19 |
| Inhoud: |
Induction of apoptosis by zinc sulfate was investigated during 96 h exposure on the cancer Hep-2 cell line. During 48 h of exposure, zinc translocated into mitochondria and stimulated production of reactive oxygen species (ROS), affected cellular GSH management and induced moderate activation of p53 and dissipation of mitochondrial membrane potential. In Zn-exposed cells, mitochondria released cytochrome c and AIF, whose translocation to the cytoplasm or the nucleus coincided with the activation of apoptosis. The use of various pharmacological inhibitors inhibiting particular apoptotic targets (antioxidants such as N-acetyl-cysteine and coenzyme Q, the caspase inhibitors z-DEVD-fmk and z-VAD-fmk, cyclosporin A and bonkgrekic acid) proved that Zn acts both directly and indirectly on mitochondria and observed apoptosis is executed by caspase-dependent and caspase-independent pathways. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 6 van 6 gevonden artikelen
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