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| Titel: |
^{18}O-Labeled lipid hydroperoxides and HPLC coupled to mass spectrometry as valuable tools for studying the generation of singlet oxygen in biological system |
| Auteur: |
Sayuri Miyamoto Glaucia R. Martinez Ana Paula B. Martins Marisa H.G. Medeiros Paolo Di Mascio |
| Verschenen in: |
BioFactors |
| Paginering: | Jaargang 22 (2004) nr. 1-4 pagina's 333-339 |
| Jaar: |
2004-12-30 |
| Inhoud: |
Decomposition of lipid hydroperoxides (LOOH) is known to generate toxic products capable to induce tissue injury. We have recently confirmed that decomposition of LOOH into peroxyl radicals is a potential source of singlet oxygen (^1O_2) in biological system. Using ^{18}O-labeled linoleic acid hydroperoxide (LA^{18}O^{18}OH) in the presence of Ce^{4+} or Fe^{2+}, we observed the formation of ^{18}O-labeled ^1O_2 (^{18}[^1O_2]) by chemical trapping of ^1O_2 with 9,10-diphenylanthracene (DPA) and detecting the corresponding ^{18}O-labeled DPA endoperoxide (DPA^{18}O^{18}O) by HPLC coupled to tandem mass spectrometry (HPLC-MS/MS). ^{18}O-Labeled alcohol and ketone were also detected providing further evidence for the generation of ^1O_2by the Russell mechanism. Similarly the reaction of LA^{18}O^{18}OH with peroxynitrite also generated^{18}[^1O_2].In conclusion, these results indicates that the use of ^{18}O-labeled LOOH associated with HPLC-MS/MS can be an useful tool to clarify mechanistic features involved in the reaction of LOOH in biological media. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 55 van 69 gevonden artikelen
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