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Details for article 3 of 3 found articles
| Title: |
Hepatocyte proliferation factors from neonatal pig liver: Purification and characterization |
| Author: |
Mineyoshi Hiyoshi Tomoichi Ohkubo Kimiyoshi Tsuji Masao Hagihara Hisao Nakasaki Masaya Mukai Hiroyasu Makuuchi Masaichi Yamamura Michio Tsuda |
| Appeared in: |
BioFactors |
| Paging: | Volume 16 (2002) nr. 1-2 pages 1-14 |
| Year: |
2002-12-28 |
| Contents: |
Two factors were found in the condition medium of neonatal pig liver fragments, which were capable of stimulating DNA synthesis in primary hepatocytes. They were named hepatocyte proliferation factor (HPF)-1 and HPF-2 and purified 1,025- and 2,580-fold, respectively. Both HPF-1 and HPF-2 seem to be anionic at pH 8.0 judged from the elution pattern of DEAE (DE52) column chromatography. HPF-1 was recovered as a non-adsorbed fraction in blue Sepharose and heparin Sepharose columns, and had a molecular weight of 26--31 kDa as estimated by gel filtration in high salt condition. Purified HPF-1 stimulated DNA synthesis of primary rat hepatocytes, but suppressed that of HepG2 cells. HPF-2 strongly bound to blue Sepharose and heparin Sepharose columns, and had a molecular weight of 71--90 kDa as estimated by SDS-PAGE under non-reduced condition. Purified HPF-2 stimulated DNA synthesis of primary rat hepatocytes dose dependently but did not suppress that of HepG2 cells. From further biological and chemical characteristics studied in this paper, HPF-1 and HPF-2 may be novel stimulating proteins for hepatocyte proliferation, although the possibility that they are already known growth factors can not be excluded without complete purification and its cloning. |
| Publisher: |
IOS Press |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 3 of 3 found articles
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