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Details for article 25 of 32 found articles
| Title: |
Selenoprotein synthesis in archaea |
| Author: |
Michael Rother Armin Resch Reinhard Wilting August Böck |
| Appeared in: |
BioFactors |
| Paging: | Volume 14 (2001) nr. 1-4 pages 75-83 |
| Year: |
2001-08-17 |
| Contents: |
The availability of the genome sequences from several archaea has facilitated the identification of the encoded selenoproteins and also of most of the components of the machinery for selenocysteine biosynthesis and insertion. Until now, selenoproteins have been identified solely in species of the genera {\it Methanococcus (M.)} and {\it Methanopyrus}. Apart from selenophosphate synthetase, they include only enzymes with a function in energy metabolism. Like in bacteria and eukarya, selenocysteine insertion is directed by a UGA codon in the mRNA and involves the action of a specific tRNA and of selenophosphate as the selenium donor. Major differences to the bacterial system, however, are that no homolog for the bacterial selenocysteine synthase was found and, especially, that the SECIS element of the mRNA is positioned in the 3' nontranslated region. The characterisation of a homolog for the bacterial SelB protein showed that it does not bind to the SECIS element necessitating the activity of at least a second protein. The use of the genetic system of {\it M. maripaludis} allowed the heterologous expression of a selenoprotein gene from {\it M. jannaschii} and will facilitate the elucidation of the mechanism of the selenocysteine insertion process in the future. |
| Publisher: |
IOS Press |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 25 of 32 found articles
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