|
| << previous | next >> |
Journal description
All volumes of the corresponding journal
All issues of the corresponding volume
All articles of the corresponding issues
Details for article 17 of 32 found articles
| Title: |
PHGPx and spermatogenesis |
| Author: |
Antonella Roveri Fulvio Ursini Leopold Flohé Matilde Maiorino |
| Appeared in: |
BioFactors |
| Paging: | Volume 14 (2001) nr. 1-4 pages 213-222 |
| Year: |
2001-08-17 |
| Contents: |
PHGPx of rat sperm mitochondrial capsule is cross-linked and inactive. The enzyme is in part released in an active form by mercaptoethanol. Treatment with H_2O_2 of reduced and solubilised capsule proteins, in the absence of any added reductant, results in: i) H_2O_2 consumption which depends on the presence of both, PHGPx activity and protein thiols; ii) protein thiol oxidation with a stoichiometry of 2 equivalents of thiol per mole of hydroperoxide and, iii) PHGPx inactivation and cross-linking. SDS-PAGE analysis of monobromobimane-labeled proteins, following incubation with H_2O_2, shows that the oxidation takes place in specific bands in the area of 20~kDa. It is concluded that the protein thiol peroxidase activity of PHGPx is responsible for cross-linking proteins in the mammalian sperm capsule and accounts for the selenium dependency of spermatogenesis. |
| Publisher: |
IOS Press |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 17 of 32 found articles
| << previous | next >> |