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Details for article 15 of 32 found articles
| Title: |
Modulation of selenoprotein P expression by TGF-β$_{1}$ is mediated by Smad proteins |
| Author: |
Mostert, Volker Dreher, Ingeborg Köhrle, Josef Wolff, Sandra Abel, Josef |
| Appeared in: |
BioFactors |
| Paging: | Volume 14 (2001) nr. 1-4 pages 135-142 |
| Year: |
2001-08-17 |
| Contents: |
Selenoprotein P (SeP) is a selenium-rich plasma protein which accounts for more than 50% of total selenium in human plasma. In this study, the effect of TGF-β1 on the expression of SeP in the human liver cell line HepG2 was investigated. Western analysis revealed a dose-dependent reduction of SeP content in cell supernatant. RT-PCR analysis of SeP-mRNA expression demonstrated a marked inhibition and a reporter gene under control of the SeP promoter was negatively regulated by TGF-β1. Smad proteins are the transcriptional mediators of TGF-β signaling. A putative Smad-binding element (SBE) is present in the SeP promoter. In electrophoretic-mobility-shift assays, TGF-β1 enhanced the binding of nuclear proteins to this SBE. Overexpression of Smad3 and 4 resulted in a downregulation of SeP-promoter activity whereas deletion of the SBE led to a loss of TGF-β1 responsiveness. We conclude that SeP expression is modulated by the binding of Smad3/4 complexes to a functional SBE in the SeP promoter. |
| Publisher: |
IOS Press |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 15 of 32 found articles
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