The mechanism of the hypochlorite-induced lipid peroxidation
Titel:
The mechanism of the hypochlorite-induced lipid peroxidation
Auteur:
Oleg M. Panasenko
Verschenen in:
BioFactors
Paginering:
Jaargang 6 (2001) nr. 2 pagina's 181-190
Jaar:
2001-04-01
Inhoud:
The article reviews data related to the role of exogenic hypochlorite (HOCl/OCl^{-}) and hypochlorite produced by myeloperoxidase catalysis in initiation of lipid peroxidation (LPO) in phospholipid membranes and human blood lipoproteins (LP). It has been shown that HOCl/OCl^{-} promotes free radical lipid oxidation in liposomes and LP that is followed by the formation of LPO products: hydroperoxides, conjugated dienes, TBARS, and fluorescent products. Water soluble reactive substances ({}^\bulletO_{2}^{-}, H_{2}O_{2}, Fe^{2+}) which can be present in the reaction mixture as a dopant are not the source of free radicals and do not participate in HOCl/OCl^{-}-induced LPO at the initiation step. The main reaction of HOCl/OCl^{-} with unsaturated lipid is probably the generation of chlorohydrins. However, this reaction is not accompanied by generation of free radicals and LPO. HOCl/OCl^{-} reacts efficiently with TBARS of aldehydic nature. It is likely that the reaction proceeds without the participation of free radicals. Among the compounds of a peroxide nature (hydro-, dialkyl-, diacyl-, alkyl-acyl-peroxide groups and epoxides) only hydroperoxides react with HOCl/OCl^{-}. This reaction is accompanied by the production of free radicals (but not singlet oxygen), probably alkoxyl radicals, which may play a role in the initiation of HOCl/OCl^{-}-induced LPO.
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