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Details van artikel 35 van 68 gevonden artikelen
| Titel: |
Interactions between macrophages and oxidized low density lipoprotein in the presence of type I collagen |
| Auteur: |
Qi Chen Enhui Wei Xiuying Chen Nan Wang Günther Jürgens |
| Verschenen in: |
BioFactors |
| Paginering: | Jaargang 6 (2001) nr. 2 pagina's 131-138 |
| Jaar: |
2001-04-01 |
| Inhoud: |
In order to investigate the influence of collagen on the interactions between macrophages and oxidatively modified low density lipoprotein (ox-LDL), type I collagen was isolated from rat tail tendon and prepared as a gel. The binding of ^{125}I-ox-LDL, ^{125}I-malondialdehyde (MDA)-LDL and ^{125}I-acetyl-LDL to collagen was higher but the binding of ^{125}I-4-hydroxynonenal (HNE)-LDL was lower than that of native ^{125}I-LDL. When mouse peritoneal macrophages were cultivated on this collagen gel, most of the modified LDL was bound to the collagen gel rather than taken up by macrophages. The amount of modified ^{125}I-LDL degraded by the macrophages decreased in the presence of the collagen gel. In the absence of gel a similar degree of reduction in degradation of modified ^{125}I-LDL by macrophages was obtained when the cells were treated with cytochalasin D, an inhibitor of non-specific phagocytosis. However, the treatment of the macrophages cultivated on the collagen gel with cytochalasin D did not influence the degradation of ^{125}I-ox-LDL and ^{125}I-HNE-LDL. These results suggest that the uptake of ox-LDL by macrophages grown on collagen gels is primarily mediated via the scavenger receptors pathway, whereas in the absence of collagen also other mechanisms of uptake are operating. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 35 van 68 gevonden artikelen
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