Mapping of a SCAR marker tightly linked to the B mating type locus of Lentinula edodes (shiitake mushroom) by tetrad analysis
Titel:
Mapping of a SCAR marker tightly linked to the B mating type locus of Lentinula edodes (shiitake mushroom) by tetrad analysis
Auteur:
A. Tanaka K. Miyazaki S. Shiraishi
Verschenen in:
Micologia aplicada international
Paginering:
Jaargang 20 (2008) nr. 2 pagina's 47-54
Jaar:
2008
Inhoud:
We isolated 132 basidiosporic strains of 33 tetrads from the outbred strains MCR14 and MCR15 of Lentinula edodes for linkage analyses. Mating tests detected crossing over between genes of the B mating-type locus during meiosis in four strains, MCR14B-121-3 (B3), MCR14B-121-4 (B4), MCR14B-130-1 (B3), and MCR14B-130-4 (B4), as monokaryons having different mating types from the parental type (B1 , B2). In MCR14B-121-3 and MCR14B-121-4, the recombination was also detected between a sequence characterized amplified region (SCAR) marker, sOPP19-560, tightly linked to the B mating-type locus and a random amplified polymorphic DNA (RAPD) marker, C02-0900t. Furthermore, sOPP19-560 mapped between the B mating-type locus and a RAPD marker, Y07-0820t
Uitgever:
MicologĂa Aplicada Internacional (provided by DOAJ)