Isolation and partial analysis of a polysaccharide-protein complex from Lactarius deliciosus
Titel:
Isolation and partial analysis of a polysaccharide-protein complex from Lactarius deliciosus
Auteur:
Jong , S. C. Ke , L. X. Yang , X. T.
Verschenen in:
Micologia aplicada international
Paginering:
Jaargang 15 (2003) nr. 2 pagina's 45-50
Jaar:
2003
Inhoud:
A crude polysaccharide-protein complex from Lactarius deliciosus fruit bodies (LDP) was extracted with hot water, precipitated, and washed with 95% ethanol. After protein removal according to the Sevag method, LDP was dialyzed against running tap water and distilled water, and further purified by Sephadex G-100 column chromatography. Gas chromatography showed that LDP is composed of glucose, galactose, mannose, and arabinose in a molar ratio of 100:14.9:6.23:1.97. According to Gel Permeation Chromatography-HPLC results, the molecular weight is 642kDa. An IR spectrum revealed coinciding polysaccharide and peptide absorption peaks, while a 1H-NMR spectrum indicated the main chain has a ß-pyranglycoside linkage. Amino acid analysis detected the presence of 17 kinds of amino acids.
Uitgever:
Micología Aplicada Internacional (provided by DOAJ)