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| Titel: |
Identification of Tilletia species using rep-PCR fingerprinting technique |
| Auteur: |
Župunski Vesna Ignjatović-Micić Dragana Nikolić Ana Stanković Slavica Jevtić Radivoje Lević Jelena Ivanović Dragica |
| Verschenen in: |
Acta biologica Iugoslavica |
| Paginering: | Jaargang 43 (2011) nr. 1 pagina's 183-195 |
| Jaar: |
2011 |
| Inhoud: |
Analyzing 167 non-processed seed samples of wheat, it was found that 145 samples (86.8 %) were contaminated with Tilletia species, while 22 (13.2 %) samples were not contaminated. By using rep-PCR fingerprinting technique, it was found that DNA isolates of T. tritici originated from Serbian wheat samples had 80 % similarity with positive control for T. tritici. One isolate shared similarity of 60% with T. tritici, T. controversa and T. laevis. It was supposed that this isolate belongs to T. bromi. Isolate of T. laevis shared a similarity of 70 % with isolates of T. tritici and T. controversa, while T. walkeri was more than 10 % similar with T. tritici, T. controversa and T. laevis. Although T. controversa and T. tritici had high percent of genetic similarity, they were clustered separately. Our results suggest that rep-PCR fingerprinting could be a useful tool for monitoring presence of morphologically similar Tilletia species in wheat production areas. |
| Uitgever: |
Društvo genetičara Srbije (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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