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| Titel: |
Optimization of Cultural Conditions for the Biosynthesis of β-fructofuranosidase by Saccharomyces cerevisiae |
| Auteur: |
Mirza Ahsen Baig Kiran Shafiq Sikander Ali Shazia Mirza Zafar Siddiq Ikram-ul-Haq |
| Verschenen in: |
Pakistan journal of biological sciences |
| Paginering: | Jaargang 6 (2003) nr. 11 pagina's 952-954 |
| Jaar: |
2003 |
| Inhoud: |
The enzyme βfructofuranosidase attacks beta-D-fructofuranoside (raffinose, stachyose and sucrose) from the fructose end. Saccharomyces GCA-II was used for the production of βfructofuranosidase by submerged fermentation. The effect of sucrose concentration and incubation time was worked out for maximal enzyme production. Sucrose at the level of 30.0 g l-1 in fermentation medium was found to exert an inducible effect on enzyme synthesis by Saccharomyces strain after 48 h of inoculation. Enzyme production increased from 107.42 U ml-1 to 118.58 U ml-1 after optimization of the cultural conditions. Specific product rate and specific growth rate were also determined for the production of βfructofuranosidase by Saccharomyces in shake flask. |
| Uitgever: |
Asian Network for Scientific Information (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 10 van 15 gevonden artikelen
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