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Details for article 24 of 30 found articles
| Title: |
PCR-based DNA Fingerprinting Analysis of Coliphages Isolated from Sewage Polluted Seawater in Alexandria |
| Author: |
Faiza A. Fattouh Hesham M. Saeed Sahar M. Wefki |
| Appeared in: |
Pakistan journal of biological sciences |
| Paging: | Volume 5 (2002) nr. 9 pages 938-942 |
| Year: |
2002 |
| Contents: |
Three coliphage isolates were propagated and purified from sewage polluted water samples. Isolates were selected only on the basis of different plaque size and shape on E. coli host (ATCC 13706). Electron microscopy of purified isolates showed isolate CPS1 to belong to family Siphoviridae whereas isolates CPS2 and CPS3 both belong to family Myoviridae. DNA fingerprinting based on restriction endonucleases analysis showed the three isolates to have no EcoRI, Pst I or Sac I sites. The isolates have different patterns of digestion with Hind III and Sal I enzymes. RAPD-PCR using 10 different primers also indicated that the three isolates have different patterns and could belong to different species of both families. |
| Publisher: |
Asian Network for Scientific Information (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 24 of 30 found articles
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