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Details for article 26 of 26 found articles
| Title: |
Transformation of Red Chilli Variety Cillibangi-2 (Capsicum annum L.) With cDNA of Cucumber Mosaic Virus Coat Protein Gene by Direct Uptake |
| Author: |
Ahmad I.B. M.N. Hanina A.M. Mahir A. Zaiton |
| Appeared in: |
Pakistan journal of biological sciences |
| Paging: | Volume 5 (2002) nr. 6 pages 683-687 |
| Year: |
2002 |
| Contents: |
The 670 bp cDNA of cucumber mosaic virus coat protein (CMV-CP) gene synthesized from purified viral RNA using a cDNA synthesis kit, was amplified by PCR using primers containing XbaI and BamHI restriction sites at the 5` and 3` ends, respectively. The cDNA fragments were cloned into pUC18, sequenced and then subcloned into plant transformation vector pBI221 to generate pBI221CP recombinant plasmid. The recombinant plasmid pBI221CP was mobilized into 12 days old chilli shoot tip explants of the variety Cillibangi-2 by a modified direct uptake method. Analysis of T1 plants derived from a T0 plant showed variability when analyzed by transient GUS assay and PCR analysis for the GUS gene and the CMV-CP cDNA. Further PCR analysis of T2 plants which were derived from eight T1 plants showed that 25 to 54% of the plants carried the CMV-CP cDNA. Repeat of the transformation method was successful in obtaining new positive plants. |
| Publisher: |
Asian Network for Scientific Information (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 26 of 26 found articles
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