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Details for article 10 of 26 found articles
| Title: |
Effects of Various Growth Regulators on Callus Formation and Regeneration in Brassica napus Cv. Oscar |
| Author: |
Muhammad Ramzan Khan Hamid Rashid Azra Quraishi |
| Appeared in: |
Pakistan journal of biological sciences |
| Paging: | Volume 5 (2002) nr. 6 pages 693-695 |
| Year: |
2002 |
| Contents: |
Hypocotyl explants from in vitro seedlings were cultured aseptically on Gamborg (B5) medium supplemented with various concentrations and combinations of dichlorophenoxyacetic acid, indoleacetic acid, naphthalene acetic acid and benzylaminopurine for callus formation and shoot regeneration. 2,4-D alone @ 2 mg/l gave the highest callus formation (96%). The results revealed that BAP @ 2 mg/l with IAA 0.5 mg/l was the most appropriate combination to produce the multiple shoots (14.5 shoots per culture). Efficient rooting was achieved on half-strength MS medium supplemented with 0.250mg/I IBA and 0.125mg/l IAA. In vitro raised plantlets were transferred to potted soil and finally to experimental fields to assess their acclimatizing potential. |
| Publisher: |
Asian Network for Scientific Information (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 10 of 26 found articles
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