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                                       Details for article 18 of 34 found articles
 
 
  Micropropagation of Annona squamosa Linn. Using Explants (Shoot Tip and Node) of Field Grown Mature Plants
 
 
Title: Micropropagation of Annona squamosa Linn. Using Explants (Shoot Tip and Node) of Field Grown Mature Plants
Author: Muhammad N. Amin
Kamrun Nahar
Firoj Ahmed
Shahrear Ahmad
Appeared in: Pakistan journal of biological sciences
Paging: Volume 5 (2002) nr. 4 pages 394-397
Year: 2002
Contents: The investigation was based on axillary shoot proliferation, culture multiplication and rooting of the in vitro generated and proliferated shoots of Annona. Different strengths of MS and MMS media showed the best result. For axillary shoot proliferation nodal explant from field grown mature plants showed the best results on MMS medium containing 0.2 mg/l BA(6-benzyl adenine) +0.1 mg/l NAA( α-naphthalene acetic acid) . The highest degree of axillary shoot proliferation from nodal explant was found 80% with 6.0±0.42 shoots per culture. Root forming performance of IBA(Indole-3-butyric acid) was found to be the best among three auxins (NAA, IAA/Indole-3-acetic acid, IBA) tested. The highest percentage of root formation was obtained with 2.00±0.21 roots per cutting on the medium containing 0.1 mg/l IBA. At the high concentration (0.1-0.2 mg/l) of all auxins callus formed from the cut margin of the micro cuttings. The regenerated shoots with well developed roots were gradually acclimatized and successfully transferred to the soil under natural conditions.
Publisher: Asian Network for Scientific Information (provided by DOAJ)
Source file: Elektronische Wetenschappelijke Tijdschriften
 
 

                             Details for article 18 of 34 found articles
 
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