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Details for article 5 of 84 found articles
| Title: |
An Improved Method for Shoot Regeneration from Calli of Indica Rice (Basmati) |
| Author: |
Hamid Rashid Kinya Toriyama |
| Appeared in: |
Pakistan journal of biological sciences |
| Paging: | Volume 3 (2000) nr. 12 pages 2229-2231 |
| Year: |
2000 |
| Contents: |
Shoot regeneration from calli of indica rice remains a limting factor for undertaking transformation experiments. In this study, callus cultures were initiated from seeds of Basmati 370, Basmati 385 and Basmati 6129. A 2,4-D concentration at 2 mg/l was found to be suitable for callus induction in Basmati 370 and Basmati 385, and was 3 mg/l for Basmati 6129 in N6 medium. Callus induction frequency ranged from 23.1%-100% for Basmati 370, 73-100% for Basmati 385 and 40-100% for Basmati 6129. Inclusion of Benzyl antiparine (BAP) in callus induction medium has a negative effect for the frequency of callus induction and callus growth. Shoot regeneration was obtained from calli in Basmati varieties between 40%-55.7% in MS medium with 3% sucrose, 3% sorbitol, 2g/l casamino acids, 5 mg/l BAP, 1 mg/l Naphthalene acetic acid (NAA) and 4 g/l gelrite. A maximum shoot regeneration was found in Basmati 370. Regeneration frequency was increased 2-7 folds by the addition of sorbitol and gelrite. |
| Publisher: |
Asian Network for Scientific Information (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 5 of 84 found articles
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