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Details for article 11 of 134 found articles
| Title: |
Characteristics of Streptomyces Flavogriseus -xylosidase and its Use in Xylan Hydrolysis |
| Author: |
El-Sawah M.M.A., Ashour Eman H. Shady, T.S.M. |
| Appeared in: |
Pakistan journal of biological sciences |
| Paging: | Volume 2 (1999) nr. 3 pages 1010-1016 |
| Year: |
1999 |
| Contents: |
-Xylosidase was induced when Streptomyces flavogriseus strain was grown in shake-culture of a mineral salt medium containing 1 per cent wheat straw hemicellulose for 3 days at 30�C. The intracellular -xylosidase was separated by precipitation with acetone, ammonium sulphate and purified on Sephadex G-150. The enzyme had an apparent molecular weight of 85 Kda as determined by SDS-gel electrophoresis. -Xylosidase showed maximum activity at pH 6.5 and temperature 40� C. It was stable at pH 6.0-8.0. Heat treatment to 45� C for 1 h did not cause any inhibition for enzyme activity. It was stable for 5 months at 4� C. It was also stable against freezing, thawing and lyophilization with 5.65 per cent loss per cycle. It hydrolyzed -nitrophenyl- -D-xylopyranoside readily with a Km. of 1.1 mM. I2, and HgCl2 were the most potent inhibitors for -xylosidase activity. Xylose was inhibitory towards the enzyme and the inhibition constant was 5.2 mM. The activity of -xylosidase and xylanase acting together was greater than the sum of the individual activities, indicating the synergism between St. flavogriseus xylanolytic components in the hydrolysis of insoluble hemicelluloses. |
| Publisher: |
Asian Network for Scientific Information (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 11 of 134 found articles
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