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| Titel: |
Improved Production of L-lysine by Over-expression of Meso-diaminopimelate Decarboxylase Enzyme of Corynebacterium glutamicum in Escherichia coli |
| Auteur: |
S. Cheraghi A. Akbarzade A. Farhangi M. Chiani Z. Saffari S. Ghassemi H. Rastegari M.R. Mehrabi |
| Verschenen in: |
Pakistan journal of biological sciences |
| Paginering: | Jaargang 13 (2010) nr. 10 pagina's 504-508 |
| Jaar: |
2010 |
| Inhoud: |
The aim of this study is over-expression of Meso-diaminopimelate decarboxylase enzyme (EC 4.1.1.20) and enhancement of L-lysine production rate. The C. glutamicum LysA gene which encodes a Meso-diaminopimelate decarboxylase was cloned in E. coli. The cloned gene was sequenced; it encodes a 445 amino acids protein with molecular weight of 47 kD. Expression of the LysA gene in E. coli resulted in an increase in Meso-diaminopimelate decarboxylase activity, correlated with the presence in sodium dodecyl sulfate-polyacrylamid gels of a clear protein band that corresponds to this enzyme. The induction of cloned gene by IPTG has been shown to have an inhibitory effect on cell growth due to over-expression of the cloned gene. A two fold increase in lysine production rate was observed after introduction of the cloned gene into E. coli. |
| Uitgever: |
Asian Network for Scientific Information (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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