|
| << vorige | volgende >> |
Tijdschrift beschrijving
Alle jaargangen van het bijbehorende tijdschrift
Alle afleveringen van het bijbehorende jaargang
Alle artikelen van de bijbehorende aflevering
Details van artikel 5 van 15 gevonden artikelen
| Titel: |
Identification of Panulirus homarus Puerulus Larvae by Restriction Fragment Length Polymorphism of Mitochondrial Cytochrome Oxidase I Gene |
| Auteur: |
G. Dharani G.A. Maitrayee S. Karthikayalu T.S. Kumar M. Anbarasu M. Vijayakumaran |
| Verschenen in: |
Pakistan journal of biological sciences |
| Paginering: | Jaargang 12 (2009) nr. 3 pagina's 281-285 |
| Jaar: |
2009 |
| Inhoud: |
Molecular identification of puerulus larvae of Panulirus homarus of the genus Panulirus from Indian coast was studied by employing Polymerase Chain Reaction, Restriction Fragment Length Polymorphism (PCR-RFLP) analysis of the mitochondrial DNA (mtDNA) Cytochrome Oxidase Gene (COI) by agarose gel electrophoresis and Denaturing Gradient Gel Electrophoresis (DGGE). The size of amplified fragment of COI gene was estimated to be approximately 1300 base pairs (bp). Single fragment amplification was recorded during different stages of the life cycle. The RFLP digestion was carried out using five different restriction enzymes (BsplI, HhaI, RsaI, TaqI and AluI). The RFLP profile of the different endonucleases, varied between 1-5 restriction types. RFLP analysis using endonuclease TaqI enabled identification of P. homarus during different stages of its life history. |
| Uitgever: |
Asian Network for Scientific Information (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 5 van 15 gevonden artikelen
| << vorige | volgende >> |