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| Titel: |
Callogenic Studies of Achyranthes aspera Leaf Explant at Different Hormonal Combinations |
| Auteur: |
Sara Kayani Muhammad Zia Sadia Sarwar Riaz -ur-Rehman M. Fayyaz Chaudhary |
| Verschenen in: |
Pakistan journal of biological sciences |
| Paginering: | Jaargang 11 (2008) nr. 6 pagina's 950-952 |
| Jaar: |
2008 |
| Inhoud: |
With the objective to promote in vitro callus induction, leaf segments of Achyranthes aspera were inoculated on basal MS medium supplemented with 3.0% sucrose and 0.8% agar with different concentrations of 2,4-D alone and in combination with NAA, BAP, IAA, IBA and Zeatin. The explants were maintained in growth room at 25 ±1 °C and 16 h light cycle. The best callus induction was obtained with 2,4-D (1.0 and 2.0 mg L-1) in combination with NAA (0.5 mg L-1). Callus induction and good texture from leaf explant was also observed at 2,4-D with BAP. On these combinations morphologically, light green, soft, compact and non-embryogenic callus (Type III callus) was observed. While morphology of callus and callogenic response was poor at 2,4-D alone or in combination with other hormones at different concentrations. |
| Uitgever: |
Asian Network for Scientific Information (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 22 gevonden artikelen
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