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                                       Details van artikel 10 van 14 gevonden artikelen
 
 
  Morphological Alteration in Mitochondria Following Diclofenac and Ibuprofen Administration
 
 
Titel: Morphological Alteration in Mitochondria Following Diclofenac and Ibuprofen Administration
Auteur: M. Moorthy
S. Fakurazi
H. Ithnin
Verschenen in: Pakistan journal of biological sciences
Paginering: Jaargang 11 (2008) nr. 15 pagina's 1901-1908
Jaar: 2008
Inhoud: This study was conducted to identify and to compare the mitochondrial morphological alterations in livers of rats treated with various doses of diclofenac and ibuprofen. Hundred and forty-four male Sprague Dawley rats were dosed with 3, 5 and 10 mg kg-1 diclofenac and ibuprofen in saline via intraperitoneal injection for 15 days. The control group was administered with saline in a similar manner. Four rats were euthanised every 3 days until day 15. While 200 mg kg-1 diclofenac and ibuprofen-treated rats (n = 4) were euthanized 10 h post-treatment. The livers were removed, cleaned and a section across the right lobe was taken and fixed in 4% (v/v) glutaraldehyde for electron microscopy analysis and the remaining samples were kept at -80°C for Western blot analysis. Five milligram per kilogram and 10 mg kg-1 diclofenac-administered rats for 15 days revealed the presence of enlarged mitochondria, irregular and ruptured mitochondrial membranes. While rats administered with 10 mg kg-1 ibuprofen also showed the presence of mitochondria with irregular membrane structure and ruptured membranes. Western blotting analysis of mitochondrial fractions revealed the expression of cytochrome c in all samples and complete absence of cytochrome c expression in the cytosolic fraction of all samples after day 15. Analysis in 200 mg kg-1 diclofenac and ibuprofen-treated groups, revealed expression of cytochrome c in both mitochondrial and cytosolic fractions. This observation indicates that both diclofenac and ibuprofen may alter the morphology of mitochondria, leading to cytochrome c release into the cytosol. Further studies needs to be conducted to investigate on the activity of the mitochondria following both treatments.
Uitgever: Asian Network for Scientific Information (provided by DOAJ)
Bronbestand: Elektronische Wetenschappelijke Tijdschriften
 
 

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