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Details van artikel 1 van 33 gevonden artikelen
| Titel: |
Amplification and Cloning of Herpes Simplex Virus Type 2 Glycoprotein G from an Iranian Isolate |
| Auteur: |
Hoorieh Soleimanjahi Marzieh Jamalidoost Fatemeh Fotouhi Zahra Meshkat |
| Verschenen in: |
Pakistan journal of biological sciences |
| Paginering: | Jaargang 10 (2007) nr. 6 pagina's 955-958 |
| Jaar: |
2007 |
| Inhoud: |
Herpes Simplex Virus type-2 (HSV-2) is the main cause of genital herpes infection. Its prevalence is increasing worldwide and varies widely with generally higher rate in developing than developed countries and urban than rural areas. HSV-2 Iranian isolate was propagated in HeLa cell line. The viral genome was extracted by phenol-chloroform and used as template in nested polymerase chain reactions (n-PCR) to amplify gG-2 gene. The amplified gene was cloned into a cloning vector (pTZ57R/T) and transformed into competent E. coli DH5a. The recombinant vector encoding the gene of interest was confirmed by restriction enzyme analysis and sequencing. |
| Uitgever: |
Asian Network for Scientific Information (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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